Figure 3.

Complementation of rpf3-1 with At1g62930 rescues the formation of mature ccmC mRNA. A, CR-RT-PCR analysis of five rpf3-1 plants containing the wild-type allele of At1g62930. Five hundred and sixty base pair products were amplified from total RNA from all of these plants and from Col and Ler wild-type plants, while no such product was amplified from the untransformed rpf3-1 mutant. A 470-bp product was generated in C24. B, A northern analysis confirmed the generation of the 1,200-nucleotide ccmC in two complemented plants. The size of the ccmC transcripts is identical in the complemented plants and in Col wild type. No mature ccmC mRNA was detected in the untransformed mutant. Lengths of size markers are given in the left margin, relevant products are indicated in the right margin.