Figure 5.

The steady-state level of the CcmC polypeptide is substantially reduced in the rpf3-1 mutant. Immunodetection analyses of total mitochondrial protein isolated from cell suspension cultures from Col wild type (Col) and the rpf3-1 mutant (rpf3-1). While in both lines a Nad9 antiserum detects the target protein in similar amounts, an antiserum raised against an oligopeptide representing the core region of the CcmC protein (Kitazaki et al., 2009) revealed a substantially reduced level of CcmC polypeptide in the mutant. Sizes of marker proteins are indicated on the right-hand side of the images.