Figure 6.

Subcellular targeting of RPF3. A hybrid protein consisting of the RPF3 mitochondrial targeting sequence (80 amino acids) and the GFP was transiently expressed in transgenic tobacco protoplasts. Fluorescence of GFP and chlorophyll and the red fluorescent protein from the sea anemone Entacmaea quadricolor (eqFP611) were visualized through different filter sets. eqFP611, which is C-terminally fused to the N-terminal part of the mitochondrial isovaleryl-CoA dehydrogenase had been established as mitochondrial marker in transgenic tobacco (Forner and Binder, 2007). A merger (GFP + eqFP611) of both images demonstrated the nearly perfect match of both patterns. Small differences were due to the rapid movement of mitochondria in the protoplast. Size bars correspond to 10 μm.