Figure 7.

RPF3 is less important for the generation of the −391/390 5′ termini in C24. A, CR-RT-PCR analysis of total RNA from two F2 hybrids obtained from a cross of C24 ($ ) with the rpf3-1 mutant (# ) and from Col, C24, and Ler wild type. Products of 470 bp were generated from total RNA of both F2 plants lacking an intact RPF3 gene and from C24. Products of 560 bp were amplified from Col and Ler. B, F2 hybrids were also analyzed by northern-blot hybridization. Relevant signals are indicted in the left. The northern probe against the CDS is shown in Figure 1D. A hybridization with an oligonucleotide complementary to the cytosolic 18S rRNA showed equal loading of the RNA gel (bottom part).