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Four CADs from cucumber exhibit activity to hydroxycinnamyl alcohols. The relative substrate activity of each recombinant CsCAD isoform against p-coumaryl alcohol, coniferyl alcohol, sinapyl alcohol, and 2-methoxybenzyl alcohol is presented as Kenz (m−1 s−1), where Kenz is calculated by dividing Kcat by Km. Statistical significance was determined using two-way ANOVA followed by Bonferroni post tests, where *** P > 0.001 refers to differences between the CsCADs for each substrate.
