Figure 7.

CsHCT activity is reduced following pectinase treatment. A, RT-PCR analysis of the mRNA expression of CsHCT in cucumber hypocotyls following pectinase treatment. Amplification of actin was used as an endogenous control. B, Quantification of p-coumaroylshikimate obtained after a 30-min incubation of total plant protein extract with p-coumaroyl-CoA following pectinase treatment. Statistical significance was determined using one-way ANOVA followed by Tukey post tests, where * P < 0.05 and *** P < 0.001.