Table I. Enzyme kinetics of recombinant CsCAD proteins.
Kenz was calculated by dividing Kcat by Km.
| Enzyme | Substrate (Alcohol) | Km | Kcat | Kenz |
| μm | s−1 | m−1 s−1 | ||
| CsCAD1 | p-Coumaryl alcohol | 889 ± 2.3 | 0.84 | 59,100 |
| Coniferyl alcohol | 16 ± 0.2 | 4.25 | 267,295 | |
| Sinapyl alcohol | 10.7 ± 1 | 2.17 | 202,402 | |
| 2-Methoxybenzyl alcohol | No activity | No activity | No activity | |
| CsCAD2 | p-Coumaryl alcohol | 1,200 ± 8 | 0.00133 | 1.11 |
| Coniferyl alcohol | 56 ± 2.3 | 2.96 | 52,884 | |
| Sinapyl alcohol | 46 ± 0.8 | 2.44 | 53,242 | |
| 2-Methoxybenzyl alcohol | No activity | No activity | No activity | |
| CsCAD3 | p-Coumaryl alcohol | 70.2 ± 5 | 1.38 | 98,809 |
| Coniferyl alcohol | 200 ± 5 | 3.58 | 50,469 | |
| Sinapyl alcohol | 70 ± 0.2 | 7.71 | 9,635 | |
| 2-Methoxybenzyl alcohol | 2.2 ± 0.1 | 61 | 26,707,407 | |
| CsCAD4 | p-Coumaryl alcohol | 489 ± 7 | 44 | 43,500 |
| Coniferyl alcohol | 66 ± 1 | 10 | 151,332 | |
| Sinapyl alcohol | 70 ± 0.5 | 1.28 | 68,996 | |
| 2-Methoxybenzyl alcohol | 3.8 ± 0.1 | 19.2 | 5,030,621 |