Figure 1.

The Bx biosynthetic pathway and deglucosylation by β-glucosidase (Glu). The enzyme catalyzing each reaction is shown in a box. Black boxes indicate glucosyltransferase (GT) and Glu examined in this study. BX6 and BX7, which catalyze sequential 7-hydroxylation and 7-O-methylation of DIBOA-Glc to DIMBOA-Glc, have been identified in maize but not in wheat or rye. The GTs we identified have been designated TaGTs and ScGT in wheat and rye, respectively. The corresponding GTs in maize have been designated BX8 and BX9.