Figure 5.

Transcript levels of TaGT and Taglu1 genes in CS and Tetra-CS. A, Northern-blot analysis of 24- to 120-h-old CS shoots. Ethidium bromide-stained rRNA was used as a loading control. B to E, Transcript ratios of four loci in 48-h-old (black bars) and 96-h-old (white bars) shoots analyzed by qRT-PCR. B, TaGTs in CS. C, Taglu1s in CS. D, TaGTs in Tetra-CS. E, Taglu1s in Tetra-CS. The chromosomal location of each locus is shown in parentheses. Note that data for each growth stage (B–E) are presented as the transcript ratio of each locus to the sum of four loci. The absence of transcripts of TaGTd and Taglu1c in Tetra-CS (D and E, respectively) is due to lack of the D genome in Tetra-CS. Data are expressed as means of triplicate experiments with sd. n.d., Not detected.