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Southern-blot analysis of barley cv Betzes (B) and Wasedori-nijo (W) probed with TaGTa and Taglu1a. Genomic DNA digests of CS wheat were included as a positive hybridization control. The barley HvACT gene whose orthologs are present in wheat (Nomura et al., 2007b) was used as a positive control for hybridization with barley DNA and for cross-hybridization between barley and wheat sequences.
