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. 2011 Sep 9;157(3):1015–1025. doi: 10.1104/pp.111.184739

Figure 4.

Figure 4.

Biochemical characterization of AtTLP18.3 phosphatase activity. A, Characterization of AtTLP18.3 for optimal pH. Optimal pH was between 3.5 and 4.5. B, Characterization for optimal temperature. Maximal activity was found at 37°C. Phosphatase activity with pNPP was used as a substrate. Phosphatase activity was assayed by incubating samples of the protein and 7.5 mm pNPP in 0.1 m sodium acetate buffer (pH 4.0). Reactions were initiated by adding purified AtTLP18.3 and incubated for 30 min at room temperature. C, Effect of divalent metal ion on the phosphatase activity. Divalent ions were removed by adding EDTA to all buffers during purification. The metal-free AtTLP18.3 reduced the specific activity of AtTLP18.3 by about 25%. However, similar activity levels were observed in the presence or absence of divalent ions. Error bars represent se.