Table II. Kinetics of phosphatase activity of AtTLP18.3.
Sample | Km | Vmax |
nmol min−1 mg−1 | ||
Substrates | ||
pNPP | 42.56 mm | 8.10 |
pSer | 47.51 mm | 14.55 |
DiFMUP | 49.56 μm | – |
Phosphopeptides | ||
Ac-(pT)AILER | 12.49 mm | 3.15 |
Ac-(pT)IALGK | 16.57 mm | 4.73 |
RRA(pT)VA | 11.89 mm | 4.97 |
KR(pT)IRR | 12.56 mm | 4.02 |
Ac-RK(pS)AGKPKN | 13.88 mm | 7.43 |