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. 2011 Aug 26;157(3):1379–1393. doi: 10.1104/pp.111.178343

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© 2011 American Society of Plant Biologists. All rights reserved.

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Figure 6. — PtiZFP1 and group A MAPKs colocalize and interact inside the nucleus. A to C, Subcellular localization of the chimeric proteins PtiMPK3-1-eYFP (A), PtiMPK6-2-eYFP (B), and PtiZFP1-eCFP (C) in N. benthamiana leaf epidermal cells. D, Anti-GFP western blot confirms the expression of fusion proteins used for localization experiments. E and F, BiFC experiments confirm the in planta interaction between nYFP-PtiMPK3-1 and cYFP-PtiZFP1 (E) and between nYFP-PtiMPK6-2 and cYFP-PtiZFP1 (F). Fluorescence and merged images are shown. Bars = 50 μm.