Figure 1.

Identification of the repression domain of HsfB1. A, Alignment of the core sequence of BRD of HsfB1 orthologs from 13 varieties of plants including Arabidopsis HsfB1. B, Schematic representation of the constructs used for transient expression analysis. Effector constructs encoded the gene for HsfB1 (white box) or for a mutant form of HsfB1 (white box with gray insert) fused to the yeast GAL4-DB (black box), as indicated. C, Relative LUC activities after cobombardment of Arabidopsis leaves with the effector and reporter constructs. The relative activity due to the pUC18 control vector was taken as 1. The asterisk indicates a P value below 0.05 between the pUC18 control and others.