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Effects of the repression domain of HsfB1 on the regulation of HS-inducible gene expression. Quantitative analysis by RT-PCR is shown for the expression of HS-inducible genes in seedlings of Pro35S:HsfB1 (black bars; B1ox), Pro35S:mutHsfB1 (white bars; mutB1ox), and wild-type (gray bars; Wt) plants treated with moderate heat (28°C) for 45 min. The expression level at the non-heat condition (23°C) in wild-type seedlings was taken as 1.
