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. 2011 Sep 23;157(3):1043–1055. doi: 10.1104/pp.111.184515

Figure 1.

Figure 1.

Comparison of subunit composition of the plastid RNA polymerase from mustard after 2D BN-PAGE and glycerol gradient centrifugation. A, Purification schemes and resulting proteins. B, PEP subunit composition obtained by 2D BN-PAGE (left, large gel, 7%–17%) and SDS-PAGE after glycerol gradient centrifugation (right, mini gel, 5%–15%). Two representative gels are shown. Total protein (150 μg) was separated and fixed, and proteins were stained with silver. Running directions of the first and second dimensions are indicated by arrows. Sizes of marker proteins separated in parallel on the same gel are given in the margins. Single subunits within the PEP complexes that gave significant hits in the databases are indicated by consecutive numbering. Corresponding proteins within the two preparations are connected by lines. Asterisks mark proteins not reproducibly found in the complexes. For identity and detailed data of mass spectrometry, see Table I and Supplemental Table S1. MALDI, Matrix-assisted laser-desorption ionization. [See online article for color version of this figure.]