Table III. ANOVA, t test, and mode of inheritance for 27 putative enzyme activity QTLs overlapping in both field experiments, 2003 and 2004.

The enzyme activity QTLs (aQTLs) are sorted according to genotype and enzyme activity trait analyzed. The ANOVA results depict the percentage variation for the factors genotype (G), environment (E), and genotype-environment interaction (GxE). An introgression was considered harboring a QTL if a significant genotype (P_G < 0.01) and a nonsignificant genotype-environment interaction (P_GxE ≥ 0.01) were observed. The results of t test analyses show the phenotypic responses as agronomical percentage values for the field experiments in 2003 [R(IL) 2003] and 2004 [R(IL) 2004]. QTLs were considered if significant changes at P < 0.05 in the same direction were observed for both field experiments. The responses of the respective heterozygote ILs [R(ILH) 2004] and associated P values from t test analysis compared with M82 are displayed. The mode of inheritance was computed and classified as described (Semel et al., 2006) as follows: R, recessive; A, additive; D, dominant; ODO, overdominant effects. The sign indicates the direction of the observed phenotypic effect of the S. pennellii allele introgressed into the S. lycopersicum M82 genome. All significant changes are depicted as follows in each cell: * P < 0.05, ** P < 0.01.

Genotype	Enzyme	ANOVA: IL 2003/2004				t Test: IL 2003/2004			Inheritance (IL/ILH 2004)
		% (G)	% (E)	% (GxE)	QTL (P < 0.01)	R(IL) 2003	R(IL) 2004	QTL (P < 0.05)	R(ILH) 2004	R(IL/H) 2004	Mode (P < 0.05)
IL-1-2	TPI	32**	4	0	aQTL	61*	68**	aQTL	35*	0.129	D+
	NAD-MDH	21**	20**	7*	aQTL	123*	49*	aQTL	10	0.147	A+
IL-1-3	TPI	22**	6	3	aQTL	94*	58*	aQTL	65**	0.854	D+
IL-2-4	TPI	36**	3	0	aQTL	52*	76**	aQTL
	PPi-PFK	52**	10**	0	aQTL	99**	164**	aQTL
IL-2-5	PPi-PFK	46**	14**	1	aQTL	102**	124**	aQTL	33	0.012	R+
IL-3-2	NAD-GlDH	56**	0	0	aQTL	204**	142*	aQTL	92	0.516	A+
IL-3-4	NAD-GAPDH	19**	9*	0	aQTL	53*	38*	aQTL
IL-4-1	PEPC	36**	0	1	aQTL	176**	104**	aQTL	109**	0.942	D+
IL-4-2	PGI	29**	1	1	aQTL	−23*	−36**	aQTL	15	0.044	R−
IL-4-4	NAD-GAPDH	40**	6*	0	aQTL	89**	80**	aQTL	17	0.028	R+
	UGP	28**	20**	1	aQTL	44*	36*	aQTL	17	0.158	A+
	FruK	14*	17**	1		136*	94*	aQTL	4	0.09	A+
IL-5-2	PPi-PFK	24**	14**	0	aQTL	48*	96*	aQTL	12	0.023	R+
IL-5-4	NAD-MDH	27**	26**	10**		144**	72**	aQTL	39	0.262	A+
IL-7-2	TPI	39**	2	2	aQTL	55*	105**	aQTL
IL-7-4	PPi-PFK	36**	14**	4	aQTL	127*	101*	aQTL	−40	0.012	R+
	ATP-PFK	23**	7	0	aQTL	63*	60*	aQTL	17	0.324	A+
IL-7-4-1	NAD-GAPDH	20**	12**	1	aQTL	38*	44**	aQTL	−36*	0.030	ODO−/R+
IL-9-2	ShkDH	15**	29**	1	aQTL	52*	53*	aQTL	−37	0.003	R+
IL-10-1	NAD-GAPDH	36**	14**	4	aQTL	42*	75**	aQTL	17
IL-10-3	Invertase	31**	1	1	aQTL	−54*	−64**	aQTL	34	0.009	R−
IL-11-3	ShkDH	20**	26**	9*	aQTL	73**	142**	aQTL	7	0.057	A+
	Invertase	26**	4	0	aQTL	−61**	−36**	aQTL	−19	0.551	A−
IL-12-3	TPI	26**	3	0	aQTL	47*	67*	aQTL	52*	0.716	D+
	PGM	17**	40**	1	aQTL	36*	32**	aQTL	3	0.099	A+
	UGP	17**	36**	3	aQTL	33**	18**	aQTL	−4	0.005	R+