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. 2011 Sep 22;157(3):1568–1579. doi: 10.1104/pp.111.181107

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© 2011 American Society of Plant Biologists. All rights reserved.

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Figure 2. — Regulation of gene transcription by RIN. Left section, mRNA levels of genes in wild type (Ac) and rin mutant during tomato fruit development measured by qRT-PCR. MG, Mature green stage. Right section, ChIP of ripening-related promoters using RIN antibodies. BK-staged rin and Ac fruits were used for the assay. Input: enrichment before immunoprecipitation; ChIPpreI: enrichment following immunoprecipitation using preImmune serum; ChIP RIN: enrichment following immunoprecipitation using serum containing RIN antibodies. Gene categories: transcription factor (A), ethylene synthesis, perception and response genes (B), cell wall modifying genes (C), carotenoid biosynthesis genes (D).