Overexpression of Ehd1 can promote Hd3a and RFT1 expression only under SD (10 h L, 14 h D) conditions. A, Flowering time was measured in transgenic plants overexpressing Ehd1 (Ehd1-ox). The average value and sd are based on more than five individuals. Data from a representative transgenic line are shown. B, Top section, immunoblots of transgenic plants around dawn. Overexpressing Ehd1 protein was detected using anti-FLAG antibody. Bottom section, ponceau S staining membrane is shown as loading controls. Shown data are the representative of two independent experiments. Parent cultivar (Taichung 65) was used as control. [See online article for color version of this figure.]