Table 2. CpG methylation profiling of 12 maternal and 3 paternal imprinting control regions.
Gene locus | Chr. | Extents of the ICRs† | Average methylation levels | ||||||
Start | End | Wild-type oocyte | DnmtLl−/− oocyte | Sperm | Blastocyst | ESC | |||
Maternally methylate imprinted genes | Nespas-Gnas | 2 | 174,119,863 | 174,126,564 | 99.3% | 5.6% | 3.9% | 38.2% | 55.9% |
Gnas (exon1A) | 2 | 174,150,877 | 174,154,638 | 95.2% | 3.5% | 4.1% | 20.4% | 7.8% | |
Peg10 | 6 | 4,696,743 | 4,699,483 | 95.9% | 6.7% | 5.5% | 31.8% | 57.1% | |
Mest | 6 | 30,684,932 | 30,689,966 | 96.5% | 2.3% | 4.2% | 30.7% | 52.6% | |
Peg3 | 7 | 6,679,787 | 6,684,257 | 98.1% | 3.0% | 2.5% | 32.1% | 42.8% | |
Snrpn | 7 | 67,147,381 | 67,151,583 | 94.1% | 35.7% | 4.6% | 34.3% | 64.9% | |
Kcnq1ot1 | 7 | 150,480,736 | 150,482,810 | 97.9% | 2.2% | 4.3% | 34.1% | 52.0% | |
Plagl1 | 10 | 12,809,697 | 12,812,131 | 99.9% | 1.3% | 7.4% | 35.4% | 53.0% | |
Grb10 | 11 | 11,925,127 | 11,927,100 | 98.0% | 1.2% | 5.3% | 38.5% | 78.7% | |
Zrsr1 | 11 | 22,871,610 | 22,874,212 | 94.1% | 5.2% | 6.8% | 34.8% | 47.0% | |
Igf2r | 17 | 12,934,169 | 12,935,816 | 99.1% | 0.9% | 3.8% | 44.2% | 53.2% | |
Impact | 18 | 13,130,435 | 13,133,510 | 97.2% | 2.4% | 6.6% | 43.1% | 38.6% | |
Paternally methylated imprinted genes | H19 | 7 | 149,764,673 | 149,771,930 | 13.5% | 0.6% | 96.5% | 40.8% | 65.5% |
Rasgrf1 | 9 | 89,767,090 | 89,775,128 | 7.4% | 0.7% | 92.0% | 25.2% | 59.4% | |
Dlk1-Meg3 | 12 | 110,762,703 | 110,773,093 | 18.9% | 0.9% | 96.8% | 32.4% | 83.1% |
†: : The extents of each region in germ cells were determined by bisulfite sequencing study [39].