Table 1. RT-PCR Primer Sequences.
Primer | Primer Length (bases) | Sequence (5′ to 3′ direction) |
CD44 P1 F | 21 | TCCCAGTATGACACATATTGC |
CD44 P2 R | 20 | CACCTTCTTCGACTGTTGAC |
CD44 v1 R | 20 | CTGTGAATTACCAAACCAGG |
CD44 v2 R | 20 | TGCTGTAGCACTAGTGCTCA |
CD44 v3 R | 20 | TCATTTGGCTCCCAGCCTGC |
CD44 v4 R | 21 | TTGTCTGAAGTAGCACTTCCG |
CD44 v5 R | 21 | GTTCCAGTTTCCTTCATAAGC |
CD44 v6 R | 20 | CCCACATGCCATCTGTTGCC |
CD44 v7 R | 21 | AATCAGTCCAGGAACTGTCCT |
CD44 v8 R | 21 | GTGTTTGGATTTGCAGTAGGC |
CD44 v9 R | 21 | GTCTTTATCTTCTTCCAAGCC |
β actin F | 20 | CGTACCACTGGCATCGTGAT |
β actin R | 21 | GTGTTGGCGTACAGGTCTTTG |
Key: F = Forward Primer, R = Reverse Primer.
For CD44v, the CD44 P1 F primer was combined with the exon-specific reserve primer.