Table 5. Summary of total CD44 and variant exons detected by immunostaining, western blotting, and flow cytometry.

Cells	Total CD44	CD44 Variant Exons
		v3	v4	v5	v6	v7	V7/8	v9	v10
Immunostaining
NOK	2–3	3	1–2S	3	2–3H	2S	3	2H	3H
DT	1–3H	2–3	0	2–3	1–2H	0	2	1–2	1–2OC
HN4	2–3	2–3H	1–2OC	1–2H	2–3H	0	2–3H	2–3H	2–3OC
HN12	2–3S	2–3OC	2–3OC/S	2–3H	2–3H	2–3S	2–3H	2–3S	2OC
HN22	-	-	-	-	-	-	-	-	-
HN8	2–3H	3	0	1–2H	2–3	0	0	2–3	2OC
HN30	2–3H	2–3	2H	2–3	2–3	0	1–3H	2–3	2
HN31	2–3	1–3	1–2S	2–3H	2	0	2OC	2–3H	2H
OSC-19	2–3	1–3	1–2H	3	3	2–3	2H	2–3	3H
OSC-20	2–3	1–2OC	0	2H	2–3OC	0	0	1–2H	0
Western Blotting
DT	A C D	C D	C	C D	C D	B C	D E	B C	D E
HN4	A C D	B D	C D	C D	D	B	D E	B C	D E
HN12	A C D	C D	C D	C D	C D	B C	D E	B C	D E
HN22	A C D	0	C	C D	D	B C	D E	B C	D E
HN8	0	B C	C D	C D	D	B C	D E	B C	D E
HN30	C D	C D	C	C D	C D	C	D E	B C D	D E
HN31	C D	C D	C	C D	C D	B C	D E	B C D	D E
OSC-19	A C D	C D	C	C D	C D	B C	D E	B C D	D E
OSC-20	A	0	C	C D	0	B	D E	B C D	D E
Flow Cytometry
DT	+ +	+ +	−	+ +	+ +	−	+ +	+ +	+
HN4	+ +	+ +	+ +	+ +	+ +	−	+ +	+ +	+ +
HN12	+ +	+ +	+ +	+ +	+ +	+ +	+ +	+ +	−
HN22	nt	nt	nt	nt	nt	nt	nt	nt	nt
HN8	+ +	+ +	+ +	+ +	+ +	+	+ +	+ +	+ +
HN30	+ +	+ +	+	+ +	+ +	−	+	+ +	−
HN31	+ +	+ +	+ +	+ +	+ +	−	+	+ +	−
OSC-19	+ +	+ +	+ +	+ +	+ +	+ +	+ +	+ +	+
OSC-20	+ +	+	−	+	+ +	−	+ +	+ +	−

Immunostaining. 0 = no staining; 1 to 3 = increasing intensity of membrane staining. ^S = sparse (<10% of cells stained); ^OC = occasional (10 to 40% of cells stained); ^H = heterogeneous (50 to 90% of cells stained); and finally, homogenous staining (90–100% of cells stained) has no superscripted symbol.

NOTE: HN22 cell line failed to grow for analysis.

Western Blotting. Band sizes: A = 90 kDa, B = 120–125 kDa, C = 130–170 kDa, D = 180–200 kDa, E = 230–250 kDa, 0 = no expression.

Flow cytometry. ++ = 50% increase in MCF, + = >15 <50% increase in MCF, − = no increase in MCF, nt = not tested.