Table 1. Primer sequences and qRT-PCR conditions for determination of cytokine expression in the spleens of dogs with visceral leishmaniasis.

Primer	Forward primer (5′ – 3′)	Reverse primer (5′ – 3′)	Genbank reference sequence	Annealing temperature
18S	CACGGCCGGTACAGTGAAAC	CCCGTCGGCATGTATTAGCT	M27358	60°
CCL19	GTAGACTGCCTGCCGTTGTGTTCA	ACTGCTGTGGCCCTTGTTCTTTG	NM_001005256.1	60°
CCL21	GCACAGGACTGTTGCCTCAA	CGGTAGCTGCGGACAACCT	NM_001005258	55°
CCR7	GGTGGTGGCTCTCCTTGTCATTTT	AGTTCCGCACGTCTTTCTTGAAGC	XM_548131.2	55°
CXCL13	GGGTGCCCAAAAAGAGAAATC	GATGGGAGGGTTCAAGCATACA	XM_845089.1	55°
LT-α	GAGACCCCAGCATCCAGAAC	AAGGCACGATCCGTGTTTG	XM_843793.1	60°
LT-β	CGGCTGGGAGGCGAAGAAAG	GGTAGGCGACGTGACAGTAGAGGT	NM_001033510.1	60°
IL10	CCACGACCCAGACATCAAGAA	ACAGGGAAGAAATCGGTGACA	U33843	60°
TGF-β	CCACTGTTCCTGTGACAGCAA	GTCGGTTCATGCCATGAATG	L34956	60°
TNF-α	AGCAAACCCCGAAGCTGAG	CGGCACTATCAGCTGGTTGTC	S74068	60°
IFN-γ	AAGGAAGACATGCTTGGCAAG	CCTGCAGATCGTTCACAGGAA	NM_001003174	60°