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Neutralisation of 10LD50/ml BoNT/A with scFv 2H8. 2H8 reacted with BoNT/A1 (43.5 pg/ml; 10 LD50/ml) and showed dose dependent inhibition of endopeptidase activity in vitro. The negative controls (43RCA and 38RCA), directed against ricin toxin, showed no inhibition at equivalent protein concentration. Results are mean with range (n = 2). BoNT A1 toxin control (10 LD50/ml): broken line. Buffer control: dotted line.
