Figure 1.

Detection of EGFR gene amplification and survival impact of EGFR copy number gain. (a) EGFR gene amplification (8.2 signals per nucleus) was observed in one patient with OGJ cancer by image-based three-dimensional dual colour FISH analysis for EGFR (red) and chromosome 7 (green). (b) Overall survival in metastatic gastric or OGJ cancer patients treated with cetuximab plus FUFOX was analysed with the Kaplan-Meier method after stratification of patients according to EGFR gene copy numbers (cut-off 4.0). The log-rank test statistical analysis indicates a statistically significant relationship (P = 0.011).