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. 2012 Jan 5;7(1):e29551. doi: 10.1371/journal.pone.0029551

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Tian et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, D) The activities of members of the let-7 family were not consistent with their expression levels. (B, E) miR-221 and miR222 activities were consistent with their expression levels. (C, F) The consistency of miRNA activity with expression levels was complex in the miR-17-92 cluster. For miR-18a, the miRNA activity was not consistent with its expression level. For miR-17-3p, miR-17-5p, miR-19a, miR-19b, miR-20a and miR-92a, the miRNA activities were consistent with their expression levels. 2^ΔCt was used to indicate miRNA expression. ΔCt = Ct_U6−Ct_miRNA. RIF, relative inhibiting fold. Error bars correspond to mean±SD (n = 3).