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EC1 and EC2 mutants alter Hh signaling in vivo. (A-E) myc-smo constructs were expressed in FLP-out clones, as indicated. Wing imaginal discs from late third instar larvae were immunostained for Ci (green), Ptc (white) or Myc-Smo (red). All images were collected at equal gains. All discs are oriented anterior to the left, posterior to the right, dorsal up and ventral down. Clones are indicated by arrows.
