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. 2012 Jan 5;7(1):e29959. doi: 10.1371/journal.pone.0029959

Figure 1. 1,25D3 response in hMSC.

Figure 1

A/B 1,25D3 stimulation of hMSC resulted in a strong increase of CYP24A1 expression in all three donors. Control cells showed no CYP24A1 expression, therefore densitometric quantification was not possible (A). The induction of OC expression is considerable and significant (**, p<0.01, student's t-test), the OPN expression is less intense. Densitometric quantification of OC and OPN expression was performed. For each donor the Fold Change was calculated by comparing the expression of 1,25D3 treated cells with control cells. The results were obtained from three independent donors and are shown as mean+SEM (B). C Immunostaining of VDR in hMSC after stimulation with 1,25D3 for 24 h. Stimulated cells show an increased localization of VDR in the nucleus, whereas the staining of the control cells is more diffuse (upper panel). Counterstaining with DAPI displays the localization of the nucleus (lower panel). One representative experiment is shown. Experiments were performed three times.