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. 2012 Jan 5;8(1):e1002467. doi: 10.1371/journal.ppat.1002467

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A. Disulfide bond prediction. The prediction was conducted using the web-based program DISULFIND (http://disulfind.dsi.unifi.it/). AA: amino acid sequence, DB_state: predicted disulfide bonding state (1 = disulfide bonded, 0 = not disulfide bonded); DB_conf: confidence of disulfide bonding state prediction (0 = low to 9 = high). B. The best predicted connectivity of disulfide bonding. The best connectivity of eight disulfide bonds was determined using the web-based program DiANNA 1.1 (http://clavius.bc.edu/~clotelab/DiANNA/). C. Dithiothreitol (DTT) treatment of SnTox1. Reaction of CS to the SnTox1 yeast culture filtrates that were treated with DTT at the indicated concentration for 2 h or 4 h at room temperature. D. Heat treatment of SnTox1. Reaction of CS to the SnTox1 yeast culture filtrates that were heated to boiling on a hot plate for 30 min or 1 h.