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. 2012 Jan 5;8(1):e1002467. doi: 10.1371/journal.ppat.1002467

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A. Expression of three pathogenesis-related (PR) genes using RT-PCR. CS (Snn1) and CS ems237-1 (snn1) leaf samples were collected at 1, 2, 4, 8, 12, 24, 36, 48, 60, and 72 h after infiltration with SnTox1 or control yeast culture filtrates. RT-PCR was conducted to compare the expression of three PR-genes (PR-1-A1, chitinase, and thaumatin) among four different interactions including CS infiltrated with SnTox1 culture filtrates (CS/+SnTox1), CS infiltrated with control culture filtrates (CS/-SnTox1), CS ems237 infiltrated with SnTox1 (ems/+SnTox1), and CS ems237 infiltrated with control culture filtrates (ems/-SnTox1). The wheat 18S gene was used as an RNA quantity control. B. Expression of three PR genes using qPCR. Comparisons were made among the four different interactions described above. The same RNA samples from RT-PCR were used in qPCR analysis. The relative expression level for each time point was normalized to the wheat 18S gene.