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. 2012 Jan 5;8(1):e1002466. doi: 10.1371/journal.ppat.1002466

Figure 9. TC-core HCV co-transports with host secretory pathway components.

Figure 9

Huh-7.5 cells were electroporated with TC-core RNA followed by transfection at 48 hours post electroporation with either ApoE-GFP (A), GFP-Rab11A (B), or GFP-VAMP1 (D) then ReAsH (red) stained at 72hpe. Otherwise, cells were incubated with transferrin-488 (C) or dextran (E) prior to imaging by confocal microscopy at 72 hours post electroporation. Shown are montages of alternating DsRed (200ms) and EGFP (200ms) exposures taken every 2 seconds. Kymographs (distance on x-axis and time on y-axis) of TC-core and cellular markers are shown to the right of the montage. Straight line on the y-axis indicates stalled transport (time change only), whereas a diagonal line indicates transport (change in time and distance). Distance traveled and time elapsed (seconds) during video acquisition are indicated on the kymograph. Arrows indicate the part of the kymograph shown in montages (left). See supplemental section for videos. F. Quantification of TC-core puncta cotransport with cellular markers as a percentage of total TC-core puncta movers.