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. Author manuscript; available in PMC: 2012 Jan 5.
Published in final edited form as: Clin Exp Metastasis. 2007 Dec 5;25(2):109–118. doi: 10.1007/s10585-007-9126-2

Figure 2. SPARC protein expression in spontaneous prostate and mammary tumors derived from SPARC−/− and SPARC+/− animals.

Figure 2

Total lysates from TRAMP prostate and MMTV-PyMT mammary tumors were probed for SPARC using two different antibodies. Top and middle panels, SPARC is present as a ~45 kDa protein in SPARC+/−, but not SPARC−/−, tissues. The lower bands in the top panel for the TRAMP samples are non-specific bands detected by the ab14071 SPARC antibody but not by the AF942 SPARC antibody (middle). Bottom, GAPDH loading controls are shown for all samples.