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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Dec 4;19(1):48–55. doi: 10.1038/nsmb.2178

Figure 5. Interactions of Ndc10-associated proteins or protein complexes in the inner kinetochore.

Figure 5

(a–d) Ni2+ affinity pull-down of 35S-labeled, in vitro translated prey proteins with purified, His-tagged bait protein, analyzed by SDS-PAGE and visualized by phosphoimaging. Each panel includes a lane loaded with 10% of the in vitro translation reaction mixture (to monitor extent of synthesis) and either in vitro translated maltose-binding protein (MBP) as a prey or purified His-tagged MBP as a bait (negative controls).