Table 1.
Data collection and refinement statistics for K. lactis Ndc10 DI–II complexed with CDEIII DNA.
| Native 1 (C2221) | SAD (SeMet, C2221) | Native 2 (P212121) | |
|---|---|---|---|
| Data collection | |||
| Space group | C2221 | C2221 | P212121 |
| Cell dimensions | |||
| a, b, c (Å) | 117.29, 147.51, 95.63 | 113.37, 148.20, 94.82 | 93.1, 99.7, 125.9 |
| α, β, γ (°) | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 |
|
peak |
|||
| Wavelength | 0.9795 | 0.9795 | 0.9795 |
| Resolution (Å) | 50 – 2.8 (2.90-2.80)a | 50 – 3.0 (3.11-3.00) | 50 – 3.6 (3.73-3.60) |
| Rsym or Rmerge | 0.113 (0.717) | 0.127 (0.603) | 0.104 (0.912) |
| I / σ I | 23.8 (2.0) | 15.5 (2.0) | 6.4 (1.4) |
| Completeness (%) | 92.6 (74.2) | 91.7 (66.8) | 99 (99.9) |
| Redundancy | 14.7 | 5.2 | 3.4 |
| Refinement | |||
| Resolution (Å) | 50 – 2.8 | 50–3.6 | |
| No. reflections | 17663 | 13133 | |
| Rwork / Rfree | 19.3 / 25.1 | 28.6 / 33.1 | |
| No. atoms | |||
| Protein | 3196 | 6392 | |
| DNA | 615 | 1211 | |
| Water | 0 | 0 | |
| B-factors | |||
| Protein | 110.87 | 122.43 | |
| DNA | 159.20 | 142.26 | |
| Water | n/a | n/a | |
| R.m.s deviations | |||
| Bond lengths (Å) | 0.005 | 0.009 | |
| Bond angles (°) | 1.147 | 1.466 |
a
Values in parentheses are for the highest-resolution shell.