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Representative photograph showing the effects of 10 mg/gm dry feed palate of NLE-induced DNA fragmentation in oocytes. (A) control oocyte showing TUNEL negative staining (arrow). (B) NLE-treatment induced DNA fragmentation as evidenced by TUNEL positive staining in oocytes showing shrinkage (arrow), (C) cytoplasmic granulation (arrow) and (D) cytoplasmic fragmentation (arrow). The irregular shape of zona pellucida is due to the proteinase K treatment during TUNEL assay. Bar = 20 μm
