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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Dec 11;19(1):40–47. doi: 10.1038/nsmb.2162

Fig. 2.

Fig. 2

Vps21 surface density and tethering activity. (a and b) Liposome tethering, measured by QLS, was examined as a function of Vps21 membrane surface density. Vps21 was loaded with (a) GTP or with (b) GDP. Insets show the onset of tethering at low Vps21 surface densities. Additional surface density data for Vps21 and Ypt7 are shown in Supplementary Fig. S1. (c and d) To test the effect of ionic strength, liposomes were decorated with Vps21-GDP or -GTP at two different surface densities, and tethering was monitored by QLS in buffers containing the indicated salt concentrations. As in (a) and (b), the indicated Vps21 surface densities are upper-bound estimates. Bars indicate mean and s.e.m. from 3 independent experiments.