Skip to main content
. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Dec 11;19(1):40–47. doi: 10.1038/nsmb.2162

Fig. 3.

Fig. 3

Vps21 interactions in trans are required for efficient tethering. (a) Schematic diagram showing two possible mechanisms of Rab-mediated tethering. (b) Schematic diagram of the bead-liposome tethering assay. (c) GTP-loaded GST-Vps21 beads were photographed after incubation for 20 or 60 min in the presence of fluorescent liposomes containing 6 mol% Ni2+-NTA-DOGS and GTP-loaded Vps21-His10. Images are representative of nine independent experiments. Scale bars = 15 μm (a) and 75 μm (b–d). (d) As in panel a inset, but without GTP-loaded Vps21-His10. (e) As in panel a inset, but liposomes were prepared without Ni2+-NTA-DOGS. (f), As in panel a, except that after a 20 min incubation, 10 mM reduced glutathione was added (left panel), or buffer without glutathione was added (right panel). Samples were then incubated for 4 min more, and photographed.