Figure 2.

Loss of one copy of c-Myb suppresses the leukemogenic potential of B cells from p190^BCR/ABL-transgenic mice.(A) Survival of BCR/ABL⁺Myb^w/d and BCR/ABL⁺Myb^w/f mice; (B) Frequency of B lymphocyte and myeloid cells in hematopoietic tissues of terminally ill mice evaluated using B cell (B220, CD43, IgM) and myeloid (GR-1) markers; (C) Frequency of B cell subsets in the bone marrow of 8 week old BCR/ABL⁺ Myb^w/f and BCR/ABL⁺Myb^w/d mice; (D) Expression of c-Myb (anti-Myb, clone 1-1, Upstate Biotechnology) in pre-B cells from BCR-ABL⁺Myb^w/f and BCR-ABL⁺Myb^w/d mice. Levels of Hsp90 were measured as control of equal loading; Colony formation of bone marrow cells from BCR-ABL⁺Myb^w/f and BCR-ABL⁺Myb^w/d mice (E) and BCR-ABL⁻Myb^w/f and BCR-ABL⁻Myb^w/d mice (F). Cells were plated at 5×10⁴ cells/plate in methylcellulose supplemented with IL-7 and colonies were scored seven days after plating. Panels represent combined results from 3 experiments, each performed in triplicate.