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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Leukemia. 2011 Sep 30;26(4):644–653. doi: 10.1038/leu.2011.264

Figure 3.

Figure 3

Requirement of Bmi1 expression in p190BCR/ABL-transformed pre-B cells. (A) Box plot shows levels of Bmi1 mRNA in BCR/ABL+Mybw/f and BCR/ABL+Mybw/d pre-B cells (n=4); (B) Western blot shows c-Myb and Bmi1 expression (anti- Bmi1 antibody, Millipore, clone F6) in pre-B cells (representative of 4 samples) from 5–7 week old BCR/ABL+Mybw/f and BCR/ABL+Mybw/d mice. Levels of Hsp90 were measured as control of equal loading; (C) Western blot shows Bmi1 expression in BCR/ABL+Mybw/f bone marrow cells lentivirally transduced with the empty vector (EV) or a Bmi1-specific shRNA. Levels of Grb2 (anti Grb2, BD Transduction Laboratories) were measured as loading control; (D) Pre-B colony formation of Bmi1 shRNA-transduced BCR/ABL+Mybw/f bone marrow cells from 8-week old mice. Bone marrow cells were transduced with the EV or Bmi1 shRNA lentivirus for 24h and plated (100,000 cells/plate) in methylcellulose supplemented with IL-7. Plates (n=6/each experiment) were scored after 10 days. 98% cells from plucked colonies were CD19+.