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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Leukemia. 2011 Sep 30;26(4):644–653. doi: 10.1038/leu.2011.264

Figure 5.

Figure 5

Expression of c-Myb and Bmi1 is required for proliferation and colony formation of Ph1 Z-181 B-ALL and SUP-B15 cells. Western blots show c-Myb and Bmi1 expression in scrambled, c-Myb or Bmi1 siRNA-transfected Z-181 (A) or SUP-B15 (B) cells; levels of Grb2 were measured for normalization. Proliferation of Z-181 (C) or SUP-B15 (D) cells after transfection with scrambled, c-Myb or Bmi1 siRNA. Transfected cells were plated at 100,000 cells/ml (in triplicate) and counted every 24 hours. Colony formation of Z-181 cells (E) or SUP-B15 (F) cells transfected with scrambled, c-Myb, or Bmi1 siRNA. Cells were plated at 100,000 (Z-181) or 75,000 cells/plate (SUP-B15 cells) in rhIL-7-containing methylcellulose. Colonies were scored after 10 days. (*p-value< 0.05, ** p-value p≤0.002).