Figure 5.

Tel1 is required for efficient elongation of T₂AG₃ tracts despite the binding of the telomerase. (A) Analysis by ChIP of the binding of Tel1–HA after HO induction, in wild-type cells. (B) Southern blots monitoring cleavage at the HO site in a tel1-Δ strain. The internal loading control (‘INT’), an uncut fragment (‘U’), and the fragment resulting from ‘U’ after induction of the HO cut (‘C’). (C) Analysis by ChIP of the binding of Est1–myc and Est2–myc after HO induction, in tel1-Δ cells. HO constructs, PCR probes, ChIP methods, and statistical analysis were as described in Figures 1 and 2, and in Materials and methods.