Figure 7.

Mec1 is not required for telomerase elongation at T₂AG₃ ends but reduces Cdc13 association and elongation by telomerase at non-TG (DSB) ends. Analysis by ChIP of the binding Est1–myc and Est2–myc (A), Rap1 (B), and Cdc13–myc (C) after HO induction, in sml1-Δ mec1-Δ strains. For Rap1 binding (B) only the differences between WT and mec1-Δ for the DSB at 3 and 4 h are significant (P=0.03 and P=0.028, respectively). For Cdc13 association (C), the statistically significant differences are indicated (^*P=0.015 and ^**P=0.006 by Mann–Whitney test). HO constructs, PCR probes, ChIP methods, and statistical analysis were as described in Figures 1 and 2, and in Materials and methods.