Figure 2.

CHO cell models with regulatable expression of SORLA and APP. (A) Parental CHO cells or CHO cells constitutively expressing human SORLA (CHO-S) or APP₆₉₅ (CHO-A) were stably transfected with Tet-off constructs for APP (pTet-APP) or SORLA (pTet-SORLA). Protein expression was detected in lysates from cells treated with the indicated concentrations of doxycycline for 48 h. (B) Quantification by ELISA of APP and SORLA expressed from the Tet-off constructs following doxycycline treatment (four independent repeats). Protein levels are indicated as percent of levels seen in the untreated conditions (set at 100%).