Figure 8.

Influence of SORLA on α- and β-secretase activities. Extracts from parental CHO cells and from cells constitutively expressing 120 nM SORLA (CHO-S) were subjected to cell-free α- (A) and β-secretase (B) activity measurement using commercially available assays (four independent repeats). (Insets) The amount of proposed α-secretases TACE and ADAM10, and of β-secretase BACE1 in both cell lines was evaluated in replicate samples by western blotting. Detection of actin was used as a loading control.