Figure 6.

Ectopic expression of STK35 enhances non-apoptotic cell death during oxidative stress. (A) HeLa cells were treated with 1 mM H₂O₂ for 1 h and then placed in fresh medium. The STK35 mRNA was measured by qPCR at the indicated times. Quantification of GAPDH was used as a control for data normalization. Values are means±s.e.m. (n=3 each). ^**P<0.01 and ^***P<0.001; Student's t-test. (B) HeLa cells were exposed to 0.5 μM STS at the indicated times and the mRNA expression levels were analysed by qPCR. Quantification of GAPDH was used as a control for data normalization. Values are means±s.e.m. (n=3 each); ^***P<0.001. (C) 293F stably expressing wtag as a control (Cont.), wtag-STK35S or wtag-STK35L1 was pretreated for 30 min in the absence or presence of 50 μM zVAD-fmk and exposed to 2 mM H₂O₂ for 2 h. Cells were stained with PI and then sorted by FACS. Values are means±s.e.m. (n=4 each) of PI-positive cells; ^*P<0.05. (D) HeLa cells transfected with indicated siRNA oligonucleotides were exposed to 5 mM H₂O₂ for 4 h. Cells were stained with PI and then sorted by FACS. Values are means±s.e.m. (n=3 each) of PI-positive cells. ^**P<0.01; Student's t-test.