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. 2011 Nov 25;31(1):14–28. doi: 10.1038/emboj.2011.423

Figure 1.

Figure 1

Depletion of IAPs with IAC induces elongated morphology and migration. (A) HeLa cells were treated with two concentrations of IAC for 16 h and the depletion of various IAPs was monitored by immunoblots. (B) Phase-contrast images of HeLa cells grown on plastic dishes coated with gelatin. The cells were seeded in the presence of DMSO or IAC (5 μM) for 15 h. (C) Elongation index of cells shown in (B). (D) HeLa cells were transfected with LifeAct to stain polymerized actin in live cells. The cells were seeded onto collagen-coated plates and the morphological changes were observed by time-lapse microscopy. (E) Morphological changes of HeLa cells embedded in thick collagen matrices 24 h after IAC treatment. (F) HeLa cells were allowed to migrate in a transwell migration chamber after IAC treatment. The migrated cells were then fixed and stained with crystal violet. Data from one representative experiment are shown. (G) The quantifications of transwell migration experiments from three independent experiments are shown (*P<0.05).