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Effect of DNA topology on DNA survival. (a) How topology of plasmid DNA (1873 bp) influences its survival during nebulization. The fraction of DNA vector of each topology over time (averaged from at least three experiments) is shown. The curves are shown fitted to a sigmodial function. (b) How topology of Minivectors (999 bp) influences its survival during sonication. The fraction of DNA was quantified the same as in (a).
