Table 1. DNA vectors used in this study.
| Length (bp) | DNA vector | Encodesa | Nebulization Survival50 (min) | Sonication Survival50 (min) | Reference or source |
|---|---|---|---|---|---|
| 281 | mv281 | — | >30 | ND | 25 |
| 336 | mv336 | — | ND | 96.13±2.95 | This study |
| 383 | mv-H1-GFPshRNA | shRNA to eGFP | >30 | ND | 26 |
| 562 | Dimer of mv281 | — | >30 | ND | 25 |
| 672 | Dimer of mv336 | — | >30 | 21.86±3.68 | This study |
| 985 | mv-H1-miR31 | Human miR-31 | >30 | ND | This study |
| 999 | mv999 | — | ND | 5.98±1.46 | 25 |
| 1109 | mv-KB4TAL-GLuc | Gaussia luciferase | >30 | ND | This study, Nanolight Technology |
| 1243 | mv-KB4TAL-mCherry | mCherry | >30 | 3.73±0.55 | This study53 |
| 1580 | mv-CMV-GLuc | Gaussia luciferase | 32.3±3.1 | 1.77±0.69 | This study, Nanolight Technology |
| 1711 | pDJC1 | — | 21.6±3.2 28.2±3.4b | ND | This study |
| 1714 | mv-CMV-mCherry | mCherry | 27.5±2.3 | ND | This study53 |
| 1873 | pQR499 | — | 22.0±1.1 24.4±1.3b | 0.85±0.10 | 54 |
| 2067 | pINV2067 | — | 19.1±0.2 | ND | Invitrogen supercoiled DNA ladder |
| 2232 | pAO | — | 16.6±3.5 | ND | 55 |
| 2679 | mv-CMV-Luc2 | Firefly luciferase | 9.6±1.5 | ND | This study, Promega |
| 2686 | pUC18 | — | 10.8±1.4 | ND | Novagen Inc. |
| 3000 | pBLUESCRIPT | — | 6.1±0.1 | ND | Stratagene |
| 3459 | pJB3.5i | attB, attP | 3.4±0.9 | ND | 56 |
| 3869 | pMC339-BbvCI | attB, attP | 4.0±0.5 | 0.17±0.04 | 25 |
| 5302 | pCR2.1-norE | norE | 1.5±0.1 | ND | This study |
Abbreviations: eGFP, enhanced green fluorescent protein; miR, microRNA; ND, not determined; shRNA, short hairpin RNA.
a
All Minivectors contain attR;25 all plasmids encode bla.
b
Propagated in LZ54.