Table 1.
[FeFe]-hydrogenase and [NiFe]-hydrogenase abundance in selected metagenomes, as measured by the number of unique shotgun sequencing reads with tblastn matches to Pfam families PF02906 and PF00374, respectively, with E-values better than 10−5.
| MG-RAST or GOLD ID | Total reads | Total Mb | [FeFe]-hydrogenase |
[NiFe]-hydrogenase |
|||||
|---|---|---|---|---|---|---|---|---|---|
| # Reads | % Reads | Per 100 Mb | # Reads | % Reads | Per 100 Mb | ||||
| ANME sediment 9-12 cm | Gs0000778 | 628,644 | 250.2 | 92 | 0.015 | 36.8 | 378 | 0.060 | 151.1 |
| Hulk Marine Vent Virome | 4448187.3 | 231,246 | 77.2 | 1 | 0.000 | 1.3 | 85 | 0.037 | 110.1 |
| Lost City chimney | 4461585.3 | 46,360 | 34.6 | 12 | 0.026 | 34.7 | 37 | 0.080 | 107.0 |
| Alvinella worm symbionts | 4441102.3 | 293,065 | 290.4 | 3 | 0.001 | 1.0 | 285 | 0.097 | 98.2 |
| Anammox bioreactor | Gs0000439 | 319,509 | 122.8 | 8 | 0.003 | 6.5 | 112 | 0.035 | 91.2 |
| Great boiling spring 77°C cellulolytic enrichment | Gs0000682 | 421,849 | 98.9 | 210 | 0.050 | 212.3 | 80 | 0.019 | 80.9 |
| Great boiling spring 85°C cellulolytic enrichment | Gs0000683 | 1,447,430 | 455.1 | 466 | 0.032 | 102.4 | 336 | 0.023 | 73.8 |
| ANME sediment 6–9 cm | Gs0000775 | 585,529 | 153.4 | 62 | 0.011 | 40.4 | 113 | 0.019 | 73.7 |
| ANME sediment 0–3 cm | Gs0000776 | 587,961 | 213.8 | 35 | 0.006 | 16.4 | 151 | 0.026 | 70.6 |
| Guerrero Negro hypersaline mat | 4440963.3–4440972.3 | 129,147 | 84.2 | 44 | 0.034 | 52.2 | 47 | 0.036 | 55.8 |
| Black soudan mine drainage | 4440282.3 | 388,627 | 38.5 | 5 | 0.001 | 13.0 | 20 | 0.005 | 51.9 |
| WHC2b spring | 4460690.3 | 118,348 | 58.5 | 44 | 0.037 | 75.2 | 23 | 0.019 | 39.3 |
| Mixed alcohol bioreactor 40°C | Gs0000872 | 2,261,434 | 491.5 | 527 | 0.023 | 107.2 | 136 | 0.006 | 27.7 |
| Fungus-growing termite | Gs0000760 | 1,736,328 | 634.9 | 138 | 0.008 | 21.7 | 149 | 0.009 | 23.5 |
| Great boiling spring surface sediment | Gs0000680 | 1,203,155 | 451.8 | 0 | 0.000 | 0.0 | 89 | 0.007 | 19.7 |
| Mixed alcohol bioreactor 55°C | Gs0000871 | 2,129,475 | 693.8 | 1004 | 0.047 | 144.7 | 125 | 0.006 | 18.0 |
| ANME sediment 3–6 cm | Gs0000777 | 496,041 | 151.5 | 6 | 0.001 | 4.0 | 19 | 0.004 | 12.5 |
| Beowulf spring (Yellowstone) | Gs0000972 | 1,141,299 | 356.7 | 0 | 0.000 | 0.0 | 27 | 0.002 | 7.6 |
| UBA acid mine drainage | 4441138.3 | 138,453 | 144.2 | 0 | 0.000 | 0.0 | 10 | 0.007 | 6.9 |
| One hundred springs plain B (Yellowstone) | Gs0000781 | 581,599 | 215 | 0 | 0.000 | 0.0 | 12 | 0.002 | 5.6 |
| One hundred springs plain C (Yellowstone) | Gs0000782 | 507,891 | 180.5 | 0 | 0.000 | 0.0 | 10 | 0.002 | 5.5 |
| Global ocean sampling expedition | 4441121.3–4442709.3 | 11,798,293 | 12,357.9 | 60 | 0.001 | 0.5 | 335 | 0.003 | 2.7 |
| Red soudan mine drainage | 4440281.3 | 334,386 | 35.4 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
| Octopus spring virome (Yellowstone) | 4441096.3 | 22,272 | 22.6 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
| TLE chimney | 4460689.3 | 135,538 | 69.4 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |