Skip to main content
. Author manuscript; available in PMC: 2012 Jan 6.
Published in final edited form as: Future Microbiol. 2011 Sep;6(9):1067–1082. doi: 10.2217/fmb.11.84

Figure 1. The rpoB gene core region target sequence and molecular beacon technology.

Figure 1

(A) The rpoB gene, the nucleotide sequence of the core region and the localization of the complementary overlapping molecular beacon probes that span the complete core region. (B) The stem-and-loop structure of a molecular beacon and onset of fluorescence following binding to a complementary DNA strand. The loop structure of the molecular beacon contains the complementary oligonucleotide probe sequence, and the fluorophore and quencher molecules are attached to the ends of the stem structure. Following hybridization, conformational change in the probe leads to separation of the fluorophore and quencher molecules and onset of fluorescence.